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The Boron & De Weer Model of Intracellular pH Regulation

journal contribution
posted on 27.08.2020, 11:25 by Rossana Occhipinti, Soroush SafaeiSoroush Safaei, Peter J. Hunter, Walter F. Boron
The classic Boron & De Weer (1976) paper provided the first evidence of active regulation of pH} in cells by an energy-dependent acid-base transporter. These authors also developed a quantitative model --- comprising passive fluxes of acid-base equivalents across the cell membrane, intracellular reactions, and an active transport mechanism in the cell membrane (modelled as a proton pump) --- to help interpret their measurements of intracellular pH under perturbations of both extracellular CO2/HCO3- and extracellular NH3/NH4+. This Physiome paper seeks to make that model, and the experimental conditions under which it was developed, available in a reproducible and well-documented form, along with a software implementation that makes the model easy to use and understand. We have also taken the opportunity to update some of the units used in the original paper, and to provide a few parameter values that were missing in the original paper. Finally, we provide an historical background to the Boron & De Weer (1976) proposal for active pH regulation and a commentary on subsequent work that has enriched our understanding of this most basic aspect of cellular physiology.

Funding

National Institutes of Health: K01DK107787 - Acid secretion and intracellular pH regulation in proximal-tubule cells

National Institutes of Health: U01GM111251 - Multi-scale modeling of gas transport through channels in living cells

National Institutes of Health: R01DK113197 - Role of RPTP-gamma in sensing and transducing acid-base disturbances in the renal proximal tubule

Office of Naval Research: N00014-15-1-2060

Multidisciplinary University Research Initiative: N00014-16-1-2535

History

Commissions

VI. Molecular & Cellular

ISSN

2744-6204

Journal Title

Physiome